Device
Part:BBa_K2936018:Design
Designed by: Weng Jiaqi Group: iGEM19_ZJUT-China (2019-10-10)
pFrmR-CAT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1664
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1664
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 907
Illegal XhoI site found at 1700 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1664
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1664
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a leak problem with the FrmR promoter.And the catalase is very sensitive with TMB.
Source
Escherichia coli